Biological Identification of Freshwater Local Fish in Bali Province

Sang Ayu Made Putri Suryani; Dewa Nyoman Sadguna; Gede Sudiarta; Kadek Johan Okan Adnyana

doi:10.55927/fjas.v4i2.18

Authors

Sang Ayu Made Putri Suryani Manajemen Sumber Daya Perairan, Universitas Warmadewa
Dewa Nyoman Sadguna Manajemen Sumber Daya Perairan, Universitas Warmadewa
Gede Sudiarta Manajemen Sumber Daya Perairan, Universitas Warmadewa
Kadek Johan Okan Adnyana Manajemen Sumber Daya Perairan, Universitas Warmadewa

DOI:

https://doi.org/10.55927/fjas.v4i2.18

Keywords:

COI, Freshwater Fish, PCR, Phylogenetic Tree, Local Fish

Abstract

This study aims to identify the molecular identification of ornamental fish in rivers in Bali using the COI gene DNA barcode. In this study, to determine the types of local fish by the PCR method of amplifying the COI gene followed by sequencing. The sequencing results showed that the length of the nucleotides produced was 615 bp to 699 bp. There are twelve species that have the potential to be ornamental fish, namely Rasbora lateristriata, Barbodes binotatus, Xiphophorus helleri, Sicyopterus microcephalus, Puntigrus tetrazona, Moenkhausia santaefilomenae, Hyphessobrycon eques, Poecilia reticulata, Xiphophorus maculatus, Pethia conchonius & etc. The nucleotide base sequences of the COI gene are uploaded on BLAST to ensure their veracity and proximity to other species. The observed species found the truth that local Balinese fish have a similarity of 70-99%.

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